Evaluación de la contaminación del aire por hongos microscópicos en el Herbario de la Universidad de San Carlos de Guatemala, Index seminum y la sección de macrohongos  del Herbario de Biología de Guatemala

K. Herrera; O. Cobar; R. Barrios; K. Pierola; W. Chamalé; C. Rosales; J. Quan; O. Fuentes; C. De	León

doi:10.54495/Rev.Cientifica.v23i1.109

Authors

K. Herrera Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala (USAC)
O. Cobar Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala (USAC)
R. Barrios Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala (USAC)
K. Pierola Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala (USAC)
W. Chamalé Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala (USAC)
C. Rosales Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala (USAC)
J. Quan Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala (USAC)
O. Fuentes Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala (USAC)
C. De León Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala (USAC)

DOI:

https://doi.org/10.54495/Rev.Cientifica.v23i1.109

Keywords:

Air quality, microscopic fungi, biocolector, herbarium, air sampling

Abstract

The indoor and outdoor quality air was determined at the University of San Carlos of Guatemala Herbarium (USCG Herbarium), the Index seminum and macrofungi section of Biology of Guatemala Herbarium (BIGU Herbarium). It was used a volumetric impactation technic through a biocolector in order to collect microscopic fungi. Sampling was carried out from October 2011 through March 2012. Further identification was performed through microscopic observation using lactophenol blue staining and also using API 20C AUX was for yeast identification. Results indicate that in USCG Herbarium environments, the highest concentration in the outdoor 3580 UFC/m3 and 300 UFC/m3 for the indoor, Index seminum 1860 UFC/m3 for the outdoor and 2300 UFC/m3 for the indoor, to microfungi section of BIGU Herbarium the highest concentration was 2790 UFC/m3 for the outdoor and 1630 UFC/m3 for the indoor.

During the months in wich the sampling was carried out, it was observed that the predominant fungi in both environments and in all sampled areas were Penicillium sp, Cladosporium sp y Aspergillus sp. It was possible to isolate fungal genera of great importance for its cellulolytic and phytopathogenic action, such as Aspergillus sp., Penicillium sp., Fusarium sp. y Paecilomyces sp. The isolation of the fungal genera made it possible to create a strain library applying the mineral oil conservation technique.

It was created a guide containing information about cleaning procedures according to biosafety infrastructure and raw materials for each establishment.

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