Evaluación del crecimiento micclial de cepas nativas de Agrocybe cylindracea (DC.:Fr) Maire, en diferentes medios de cultivo y pH

C. Reyes; M. Bran; O. Morales

doi:10.54495/Rev.Cientifica.v21i2.133

Authors

C. Reyes Departamento de Microbiología, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala (USAC)
M. Bran Departamento de Microbiología, Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala
O. Morales Departamento de Microbiología, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala (USAC)

DOI:

https://doi.org/10.54495/Rev.Cientifica.v21i2.133

Keywords:

Mycelial growth, fruiting bodies

Abstract

Agrocybe cylindracea is a dingus that is consumed in the town of Tecpán Guatemala. Chimaltenango; San Juan Ostuncalco. Quetzaltenango and villages Escuachil and Vista Hermosa Sacatepéquez San Antonio, San Marcos. In the present study five strains were used of A. cylindracea Guatemalan, determining colonial morphology and mycelial growth in different culture media and pH. in order to stablish the best condition for cultivation in the laboratory as a preliminar} step to obtaining fungal biomass, and seed production of fruiting bodies. It was determined that all colonies of the strains tested showed the morphological characteristics reported in the literature for A. cylindracea (except for the amount of aerial mycelium which was abundant) in all media and pH studied. Tn evaluating mycelial growth as culture media using potato dextrose agar (PDA), malt extract agar (EMA), and cornmeal agar and yeast (CMYA), adjusted to pH 5, 5.5 and 6 and incubated at a temperature of 26 0 C, it was determined that the culture medium al pH 6 EMA favored the growth of most strains. In assessing the interactions between strain, culture medium and the pH was observed that the mycelial growth combined with greater strains were obtained with 638.08 and 58.01 in the media PDA and EMA at pll 6. Additionally, strain EMA 638.08 in the Medium EMA can have good growth at pH 5 and 5.5. As a consequence of the results, we recommend die cultivation of both strains under the conditions indicated for mycelial biomass production as a precursor to rhe production of inoculum and fruiting bodies.

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