Biocidal Activity Of Four Plants For Medicinal Use In The Laguna De Lachuá National Park

Abstract

The main objective of this research was to determine the biocidal activity of four plants for medicinal use in the Laguna de Lachuá National Park (PNLL), using standardized methods for bacteria, fungi, yeasts, protozoa, larvae, and cytotoxicity against Artemia salina. The plants under study were selected from a total of 209 reported in the ethnobotanical survey carried out by Cleaves in the year 2000 (1). The selection criteria included not having been studied previously, according to the N APIRALERT rat base, and being native to the region under study (2-7). Prior to the respective biological analyses, the ethanolic extracts of the plants under study were obtained by precolation with 95% ethanol and vacuum evaporation. It was shown that the extracts of two of the four plants had positive biocidal activities, these being: Piper aeruginosibaccum with activity against Staphylococcus aureus, Mycobacterium smegmalis and Bacillus subtilis, up to a concentration of 0.5 mg/ml, positive activity against epimastigotes of Trypanosoma cruzi a a concentration of 1 mg/ml and cytotoxic activity against nauplii of A. salina at a concentration of 0.69 mg/ml. The plant specimen Hyptis verlicillata, showed activity against epimastigotes of 1. cruzi at a concentration of 0.92 mg/mL and against promastigotes of. Leishmania brazíliensis at a concentration of 0.81 mg/mL, Cissampelos tropaeolifolia and Passiflora coriacea species did not show any bioactivity. These data were not known and constitute the basis for future research that seeks to explore in greater depth the biological properties demonstrated. They can also be used to scientifically guide the use of plants for medicinal purposes.