Evaluación de la calidad genética de las ratas Wistar  Kyoto del bioterio de la Facultad de Ciencias Químicas y  Farmacia por marcadores moleculares microsatélites

Rudy Marroquin Rosales; Dulce Saldaña Santiago

doi:10.54495/Rev.Cientifica.v27i2.67

Authors

Rudy Marroquin Rosales School of Pharmaceutical Chemistry, University of San Carlos of Guatemala
Dulce Saldaña Santiago Research Department Biotecnología Farmacéutica y Asesoría en Nutrición

DOI:

https://doi.org/10.54495/Rev.Cientifica.v27i2.67

Keywords:

PCR, polymorphisms, genealogy, genetic quality control, experimental animals

Abstract

Genetic quality control of experimental animals used in laboratory animal centers must be priority, to ensure that studies have reproducibility and also scientific validity. This research aimed to evaluate the genetic purity of the Wistar Kyoto rats at bioterio of the Faculty of Chemistry Sciences and Pharmacy at the University of San Carlos of Guatemala, “Dr. Amarillis Saravia Gómez”, which never had been genetically characterized. The D1Mgh6 and D17Mit3 microsatellites were analyzed because they have a high grade of polymorphism and allows to have a general vision of genetic characteristics of the strain, the analysis was performed by the polymerase chain reaction technique (PCR). The results of the genetic analysis showed that the colony of Wistar Kyoto rats corresponds to the estimated size for the microsatellite D17Mit3 (201 bp) as the genomic database rat and reported by Charles River Laboratories and the NIH. However, It doesn’t correspond to the estimated size of the microsatellite D1Mgh6 (147 bp). The WKY strain has been manage with an inbreeding method, that was confirmed by the results because D17Mit3 and D1Mg6 showed an homogenic and homoallelic condition, so these experimental animals can be used in trials as a inbreed model, because do not have the genetic characteristics expected for the WKY breed.

Downloads

Download data is not yet available.

References

Benavides, F., & Guénet J.L., (2003). Manual de genética de roedores de laboratorio: Principios básicos y aplicaciones. Madrid: Universidad de Alcalá.

Bryda, E. C., & Riley, L. K. (2008). multiplex microsatellite marker panels for genetic monitoring of common rat strains. Journal of the american association for laboratory animal science, 47(3), 37-41.

De Jesús, R., Moreno, N., & Martínez, J. A., (2005). Ensayo de dos métodos de extracción de ADN de ratón para ser usado en el control genético de ratones consanguíneos mediante la reacción en cadena de la polimerasa. Revista científica. XV (2) ,134-140.

Deschepper, C.F., Prescott, G., Hendley, E.D., & Reudelhuber, T.L., (1997). Genetic characterization of novel strains of rats derived from crosses between wistar-kyoto and spontaneously hypertensive rats, and comparisons with their parental strains. Journal of laboratory animal science. 47(6), 638-646.

Howes, L.G., & Louis, W.J., (1990). Genealogy of the spontaneously hypertensive rat and Wistar-Kyoto rat strains: Implications for studies of inherited hypertension. Journal of cardiovascular pharmacology. 16(7), 1-5. https://doi.org/10.1097/00005344-199006167-00002 DOI: https://doi.org/10.1097/00005344-199006167-00002

Martínez, C., Osorio, M., Rodríguez, E., & Lolas F. (2007). El animal como sujeto experimental: spectos técnicos y éticos. Chile: Universidad de Chile.

Mashimo, T., Voigt, B., Tsurumi, T., Naoi, K., Nakanishi, S., Yamasaki, K., … Serikawa, T. (2006). A set of highly informative rat simple sequence length polymorphism (SSLP) markers and genetically defined rat strains. BMC Genetics, 7(19),1-8. https://doi.org/10.1186/1471-2156-7-19 DOI: https://doi.org/10.1186/1471-2156-7-19

Romero, A., Díaz, A., Rendón, B., & Rocha, M. (2014). Herramientas moleculares aplicadas en ecología: Aspectos teóricos y prácticos. México: Secretaria de medio ambiente y recursos naturales.

Rodríguez, N. (2008). Monitoreo genético y fenotípico en ratas no consanguíneas Sprague-Dawley producidas en el Bioterio de la Universidad de los Andes. (Tesis de licenciatura). Facultad de Ciencias. Universidad de los Andes, Venezuela.

Sambrook, J. & Russell, D. (2001). Molecular cloning a laboratory manual. New York: Cold spring harbor laboratory press.

Serikawa, T., Kuramoto, T., Hilbert, P., Mori, M., Yamada, J., Dubay, C. J., … Beckmann, J. S. (1992). Rat gene mapping using PCR-analyzed microsatellites. Genetics, 131(3), 701-721. https://doi.org/10.1093/genetics/131.3.701 DOI: https://doi.org/10.1093/genetics/131.3.701

The rat genome database (2015). Genomic, phenotypic and environmental variations and disease. Nucleic acids research. Wisconsin: Medical college of Wisconsin. Recuperado de http://rgd.mcw.edu/.