Molecular characterization and antibiotic susceptibility of Mycobacterium tuberculosis strains isolated from patients attending an urban hospital in Guatemala
DOI:
https://doi.org/10.54495/Rev.Cientifica.v16i1.242Keywords:
Molecular characterization, antibiotic susceptibility, Mycobacterium tuberculosis strains, patients attending an urban hospital in GuatemalaAbstract
The objectives of this research were the characterization of genetic patterns, antibiotic susceptibility and epidemiological factors associated with infections caused by strains of M. tuberculosis. For this study, strains were obtained from patients who attended the San Juan de Dios General Hospital from 2000 to 2002 by reviewing medical records.
For molecular characterization, the subtyping method called Double Repeated Elements-Polymerase Chain Reaction (DRE-PCR) was used; antibiotic susceptibility was performed through the modified method of proportions. Epidemiological information was obtained through review of medical records. The data was entered into databases and analyzed using the statistical program SPSS version 10.
In the molecular analysis, it was found that 74% of 246 mycobacterial strains evaluated presented grouped genetic patterns and 26% unique patterns, which indicates the simultaneous occurrence of recent infections and reactivations. In the antibiotic susceptibility evaluation, 81% of 95 strains were susceptible to the four antituberculous drugs (rifampin, isoniazid, ethambutol, and streptomycin); 5.3% presented resistance to at least one drug and 6.7%' were classified as multi-resistant, the latter strains being isolated from lung samples. Drug resistance was observed in 7 strains with clustered genetic patterns, from people infected or not with HIV. residents of endemic areas and some who were deprived of liberty.
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Copyright (c) 2003 B. Samayoa, A. Xet Mull, T. Velásquez , D. Lau, L. Paz
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