Producción de Poli(β-hidroxibutirato) utilizando Glicerol como Fuente de Carbono

F. Ramos; V. Estrada; A. Marcelo; M. Villar; M. Diaz

doi:10.54495/Rev.Cientifica.v24i1.103

Authors

F. Ramos Planta Piloto de Ingeniería Química, PLAPIQUI (UNS-CONICET), Departamento de Ingeniería Química, UNS, Camino "La Carrindanga" Bahía Blanca
V. Estrada Planta Piloto de Ingeniería Química, PLAPIQUI (UNS-CONICET), Departamento de Ingeniería Química, UNS, Camino "La Carrindanga" Bahía Blanca
A. Marcelo Planta Piloto de Ingeniería Química, PLAPIQUI (UNS-CONICET), Departamento de Ingeniería Química, UNS, Camino "La Carrindanga" Bahía Blanca
M. Villar Planta Piloto de Ingeniería Química, PLAPIQUI (UNS-CONICET), Departamento de Ingeniería Química, UNS, Camino "La Carrindanga" Bahía Blanca
M. Diaz Planta Piloto de Ingeniería Química, PLAPIQUI (UNS-CONICET), Departamento de Ingeniería Química, UNS, Camino "La Carrindanga" Bahía Blanca

DOI:

https://doi.org/10.54495/Rev.Cientifica.v24i1.103

Keywords:

Hibiscus sabdariffa, Roselle, Anthocyanins, Antioxidant, Hypertension, Dyslipidemia, Hyperuricemia

Abstract

In this work, we carry out a design and a preliminary dimensioning of a poly(β-hydroxybutyrate) (PHB) plant, based on microorganisms and using glycerol as raw material. The process comprises three main sectors: a) raw material purification, where the glycerol stream (coming from a biodiesel production plant) is purified by eliminating methanol; b) fermentation, where the growth of the microorganisms (Bacillus megaterium) takes place and the production of PHB occurs. In this point, a model of the specific growth rate for B. megaterium is implemented within a control vector parameterization approach; c) biopolymer separation, which includes the extraction process, where cellular walls are exposed to lyses and a purified product, is obtained.

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References

Aspen Plus version 7.2, Aspen Technology, Inc., Cambridge, Massachusetts, USA, 2010. Cámara argentina de recursos renovables. Renovables. Recuperado de : http://www.cader.org.ar/informes-y-estudios/evolucion-del-mercado-de-biocombustibles-en-la-argentina.htm [citado el 29 de Marzo de 2012].

López Jiménez, J. A (2011) Biopolímeros de interés industrial. Síntesis y caracterización de polihidroxibutirato (PHB). Bahia Blanca, Argentina. Universidad Nacional del Sur.

López, J. A., Bucalá, V. & Villar, M. A (2010) Application of Dynamic Optimization Techniques for Poly(Beta-hydroxybutyrate) Production in a Fed-Batch Bioreactor. Industrial & engineering chemistry research, 49, 1762-1769. https://doi.org/10.1021/ie9006547 DOI: https://doi.org/10.1021/ie9006547

López, J. A., Naranjo J. M., Higuita J. C., Cubitto M. A., Cardona C. A. & Villar M. A (2012) Biosynthesis of PHB from a Nex Isolated Bacillus megaterium Strain: Outlook on future developments with endospore forming bacteria. Biotechnology and bioprocess engineering, 17, 250-258. https://doi.org/10.1007/s12257-011-0448-1 DOI: https://doi.org/10.1007/s12257-011-0448-1

Posada, J. A., Cardona, C. A. & Rincón, L. E.(2010) Sustai-nable biodiesel production from palm using in situ produced glycerol and biomass for raw bioethanol. 32nd Symposium on biotechnology for fuels and chemicals. Clearwater Beach, Estados Unidos, Abril.

Posada, J. A., Naranjo, J. M., Lopéz, J. A., Higuita, J. C. & Cardona, C. A (2011) Design and analysis of PHB production processes from crude glycerol, Process biochemistry, 46, 310-317. DOI: https://doi.org/10.1016/j.procbio.2010.09.003

Ramos, F. D., Estrada, V., Diaz, M. S. & Villar, M. A (2013) Optimización de la producción de Poli(-hidroxi-butirato) a partir de Glicerol. 13º Simposio argentino de informática. Córdoba, Argentina, Septiembre Renewables Global Status Report, 2013 Update, p. 30. Recuperado de : http://www.ren21.net/Portals/0/documents/Resources/GSR/2013/GSR2013_lowres.pdf

Ulrich, G. D. & Vasudevan, P. T. (2004) Short-Cut Equipment Design. En: Chemical Engineering Process Design and Economics: A Practical Guide (Ulrich y Vasudevan Eds.). McGraw-Hill, Durham, 89-324.